[3dem] DDD on 120kV TEM equipped with LaB6

[Benjamin Bammes]

Hi Mohammad,

I collected some cryo-EM data with a DE-12 on a LaB6 120 kV TEM a couple
years ago (see attached slides). Using about 60 images collected in less
than an hour, I got a 10 Angstrom reconstruction of Mm-Cpn. The combination
of the high SNR of the DE-12 and it's high speed for doing motion
correction seemed to make a big difference (note that very few 120 kV LaB6
reconstructions at that time had reached 15 Angstroms resolution, let alone
10). I've always been interested to see if someone could push this further,
since we clearly saw Thon rings up to 6 Angstroms or so. So I think this is
quite promising, providing the direct detector has sufficient dynamic range
for lower energy electrons and high-speed dose fractionation for motion
correction on these less stable instruments.

Note that the DE-12 camera has since been updated and is now the
DE-DirectView.

(Acknowledgment to Wah Chiu who was our collaborator on this short
proof-of-concept experiment.)

----

*Benjamin Bammes, Ph.D.*
*Director of Applications & Product Development*bbammes at directelectron.com
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On Thu, Mar 21, 2019 at 9:01 AM Mazhab-Jafari, Mohammad <
Mohammad.Mazhab-Jafari at uhnresearch.ca> wrote:

> Dear 3DEM community,
>
>
>
> I would like to know if anyone has used a K2 or any other direct detector
> device cameras on a 120kV TEM equipped with a LaB6 emitter? If so what
> resolution range can be expected for 3D reconstruction of a well-behaved
> protein (~1MDa in size) with single particle image analysis method?
>
>
>
> Any insights will be appreciated.
>
>
>
> Kind regards,
>
> Mohammad.
>
>
>
>
>
> -----------------------------------------------------------------------
>
> Mohammad Mazhab-Jafari, Ph.D.
>
> Assistant Professor, Department of Medical Biophysics
>
> University Health Network
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