[3dem] 3D classification

[Eric Hanssen]

Hi all,
Before I commit some computer resource to a job I'd rather know if it is doable.

I have 200,000 particle of a 600kDa protein, a current map at 3.4A that looks very nice, I do not need a better resolution for now. I have a drug (dipeptide) attached to the protein but  only a subset of the protein has the drug bound. Is it possible with a 3D classification to separate such a small difference? I don't mind which software, relion, cryosparc, eman2 ....

Or is there a better way, eg. 3D classification of a very small box size centered around the binding site?

Cheers
Eric


-----------------
Assoc. Prof Eric Hanssen

Head - Advanced Microscopy Facility
Honorary Principal Fellow - Department of Biochemistry and Molecular Biology

President Australian Microscopy and Microanalysis Society

Bio21 Molecular Science and Biotechnology Institute
30 Flemington Road - The University of Melbourne - Victoria 3010 - Australia
email: ehanssen at unimelb.edu.au<mailto:ehanssen at unimelb.edu.au> | Office: +61 3 83442449 | Microscope: +61 3 83442509
Web: www.microscopy.unimelb.edu.au<http://www.microscopy.unimelb.edu.au/>

[bio21]   [amms-logo]



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