[3dem] How to make grids positively charged during or after glow discharge.

[Liz Kellogg]

Maybe try coating the grid with PEI? It's something that the Nogales lab
does quite a bit, it's described in the supplement of this paper
https://www.ncbi.nlm.nih.gov/pubmed/30442764

HTH
Liz

On Wed, Jul 3, 2019 at 2:06 PM Ross, Bradford <bradford.ross at botany.ubc.ca>
wrote:

> Hello Everyone,
>
>
> I'm currently trying to image some lipid nanoparticles which have a
> positive charge on their outer surface. Of course, since normal glow
> discharge treatment typically yields a negative charge on the grids, the
> particles are all sticking to the lacey carbon support instead of remaining
> suspended in the vitreous ice layer.
>
>
> I have heard that Magnesium Acetate can be used to switch the charge from
> negative to positive after glow discharging, but can't seem to find any
> protocols for exactly how to treat the grids with it.
>
>
> Does anyone have a protocol for the use of Magnesium Acetate, or any other
> suggestions on how to flip the surface charge of the grids to positive? Or
> other creative solutions I'm not thinking of?
>
>
> Cheers,
> Bradford Ross
>
> Electron Microscopy Technician
> BioImaging Facility
> University of British Columbia
> Cunningham Building Rm. 64
> 2146 East Mall
> Vancouver, B.C.
> V6T 1Z4
>
> phone 604-822-6996
>
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>
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