[3dem] freezing conditions in high salt?

[frangube]

Dear all, 
I was wondering if anyone has experience in obtaining nice, thin ice by freezing a protein in 300mM NaCl (and 20mM Hepes pH 8, no detergent) using a Leica EM GP. 
If anyone has suggestions I would really appreciate it!

Thanks in advance.

Francesca

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Dr Francesca Gubellini
Unité Microbiologie Structurale
Institut Pasteur
25 rue du Docteur Roux  
75015 Paris
France   
Tel: +33 (0)1.40.61.35.31
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