[3dem] cryo-holder pumping station

[Mariena Silvestry Ramos]

Hi Daniel,


We built our own at Cornell because our facility has a ton of users that do multiple experiments (ST cryo, DT cryo, DT room temp, tomography, needle tomography, etc). I can send you the parts we purchased (but they're based out of US companies, though, along with drawings and pictures. It cost us <$20K USD to put it together, and can expand to up to 9 holders. Maintenance is very similar to Gatan's dry pumping station (basically the same parts, a turbo, a cold cathode gauge and butterfly valves, and those are the most expensive parts). Alternatively, as some labs move to more automated stuff, they may be willing to part with their stations and you can probably negotiate something. If $ is not a limiting factor, by all means, go with the Gatan dry pumping station. My experience has been that they're easy enough to maintain, and Gatan's engineers, at least in the US, are very responsive, super helpful and will even send you instructions on how to do some of the basic maintenance yourself.


Best,


Mariena


________________________________
From: 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of Levy Daniel <Daniel.Levy at curie.fr>
Sent: Wednesday, November 28, 2018 11:33 AM
To: 3dem at ncmir.ucsd.edu
Subject: [3dem] cryo-holder pumping station


Dear All

We want to renew our pumping station for a 626 cryo-holder. We have a Varian Minitask Plus that worked well but it is not available anymore.

Do you have any suggestion ?

Best daniel



Daniel Levy

Team Molecular Microscopy of Membrane

UMR 168 Institut curie, France

https://science.institut-curie.org/team-levy

phone 33 156246782




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