[3dem] Micrographs manual sorting relion3

[Daniel Asarnow]

The fastest way to manually go over micrographs is to create 4x binned PNG
copies using e2proc2d.py, and then go through them using eog (eye of gnome)
or another image viewer that lets you advance through images in file name
order and delete them (in eog, arrow keys and delete key). Then move all
the images for which the PNG does not exist. You can go through 1000
micrographs per hour in this way.

If you want to use statistics, I recommend making a plot of CTF figure of
merit (y) vs. CTF fit resolution (x), usually the "good micrographs" are in
a population in the bottom left with good fit resolution and not *too high*
figure of merit (usually crystalline ice).

-da

On Thu, Nov 22, 2018 at 9:18 AM Julien Bous <julien.bous at etu.umontpellier.fr>
wrote:

> Dear All,
>
> I wish to realise a sorting of my realigned micrographs in Relion3, in
> order to get rid of broken ice area. I wanted to do it in the subset
> selection mode but I was not able to set a lowpass filter in this mode
> and it was challenging to asses the micrographs quality. I thougt to do
> it in the manual picking mode or manually, with eman , but in this way I
> can just display the micrographs one by one and it'll be very time
> consuming. Therefore if anyone  knows how to realise this task
> effectively I'll be glad to know your opinion.
>
> Cheers,
>
> Julien
>
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