[3dem] Vacuum pump for glow discharge purposes

[John Rubinstein]

Dear Lena,

We’ve used the Pelco easiGLOW extensively with the standard pump for the past four or five years. It works nicely and I think it is great system. My only criticism is that if users aren’t careful it is easy to chip the bottom edge of the bell jar when removing it or putting it in place, which makes the bell jar incapable of making a seal (I think a replacement bell jar is just a few hundred dollars).

Best,
John
-- 
John Rubinstein
Molecular Medicine Program
The Hospital for Sick Children Research Institute
686 Bay Street, Rm. 20-9705
Toronto, ON
Canada
M5G 0A4
Tel: (+001) 416-813-7255
Fax: (+001) 416-813-5022
www.rubinsteinlab.org

> On Mar 1, 2018, at 9:29 AM, Lena Maria Muckenfuss <l.muckenfuss at bioc.uzh.ch> wrote:
> 
> Dear list members, 
> 
> we are about to purchase a "easiGlow” glow discharge system from PELCO for single-particle cryo-EM purposes and were wondering if anyone has experience with the supplied GD4 rotary vacuum pump, which is not oil-free. Are the concerns of oil leaking into the chamber true and is it worth purchasing an oil-free pump (e.g. Edwards XDS6i) instead? We would be very thankful about any experience you are willing to share. 
> 
> Best wishes, 
> 
> Lena
> 
> ----------------------------------------------
> Lena M. Muckenfuss
> PhD student
> Group of Prof. Martin Jinek
> University of Zurich 
> Department of Biochemistry
> Winterthurerstr. 190
> CH-8057 Zurich
> +41 44 635 555 | 44-L-82
> ----------------------------------------------
> 
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