[3dem] Any experiences with SMA extracting proteins in native membrane?

Matthews-Palmer, Teige Rowan Seal t.matthews-palmer14 at imperial.ac.uk
Tue Jun 12 09:04:01 PDT 2018


Dear 3DEMers,

I only recently became aware of SMA co-polymers for solubilising membrane proteins in a ‘native bilayer’ environment https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5593810/ - and to be honest was a bit shocked I hadn’t heard of this option before.
Supposedly you can directly solubilise your target with some amount of native membrane, which seems an improvement over MSP nanodiscs and salipro which require you to transfer your detergent-solubilised protein in to the lipid construct.

I wonder how many of you have experience with this. I’d love to hear anyone’s first hand experience and any caveats that may be hard to find in the literature - like solubilisation efficiency, dependence on your target’s density in your membranes, any difficult behaviour with plunge freezing on holey support foils? Importance of testing different co-polymer average sizes to suit your target?

Thanks & best wishes,
Teige
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