[3dem] [ccpem] Converting MRC to tiff

[Nicolas, William (William)]

Hey Yehuda,

As Marin is saying this isn’t going to save any space to convert MRC in TIFF. However, if you happen to want to perform this task, this is how you can do it:

Both methods involve ImageJ. You can either use Bioformat plugin to open .mrc although I don’t like doing that. Instead there is another plugin called U759_inputoutput.jar (http://www.cmib.fr/en/download/softwares/input-output.html) that allows you to open .mrc with ImageJ. Then all you got to do is save as > TIFF.
Check if metadata are kept by doing so.

Cheers,

William Nicolas, HHMI Postdoc.

Jensen Laboratory - Meyerowitz Laboratory
Division of Biology and Biological Engineering
1200 East California Blvd
Postal code: 156-29
California Institute of Technology
Pasadena, CA 91125, USA





Le 3 juin 2018 à 03:18, Marin van Heel <marin.vanheel at googlemail.com<mailto:marin.vanheel at googlemail.com>> a écrit :


Dear Yehuda Halfon

The em2em converter (Image-Science.de<http://image-science.de/>) should do the trick but storing (4-bit/8-bit?) MRC movies in tiff is not a good idea: very few programs can handle tiff stacks. Moreover if the TIFFs are not compressed you will not necessarily win any space. You are probably best off using a standard loss-less compression program ("zip"), and convert them back when you need it again. You must always keep your original raw data "forever" in a loss-less form.

Marin van Heel


On 03/06/2018 06:02, Yehuda Halfon wrote:
Hi there,

We have a bunch of MRC movie files the are eating at out storage, and since more are coming I was wondering if there is a good way to convert them into tiff to save space?

I know that the best way is to save them directly as tif from EPU/ serialEM and that is what we will to in the future. But we need to find a solution to the ones we have now.

Thanks,

Yehuda Halfon

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