[3dem] [ccpem] 2D centering of particle images

[Marin van Heel]

Dear Steven Wang,

Alignment is more of a statistical issue affecting the whole data set 
than something that should be done on an individual (noisy) 
particle-by-particle basis.
See, for example, our: "Alignment By Classification" (ABC) paper: 
http://journals.iucr.org/m/issues/2017/05/00/kf5002/index.html

But our IMAGIC-4D software does of course have various options to do it 
particle-by-particle if that is what you insist you need for testing.

Cheers

Marin van Heel



On 23/07/2018 19:29, Wang, Steven wrote:
> Dear all,
>
> Do you know any software available for aligning the center of geometry of each selected particle to the center of the particle-selection box? I need this centering for testing some algorithms, not for the regular cryo-EM map construction workflows. Thanks.
>
> Steven Wang
>

-- 
==============================================================

     Prof Dr Ir Marin van Heel

     Laboratório Nacional de Nanotecnologia - LNNano
     CNPEM/LNNano, Campinas, Brazil

     tel:    +55-19-3518-2316
     mobile  +55-19-983455450 (current)
     mobile  +55-19-981809332
                  (041-19-981809332 TIM)
     Skype:  Marin.van.Heel
     email:  marin.vanheel(A_T)gmail.com
             marin.vanheel(A_T)lnnano.cnpem.br
     and:    mvh.office(A_T)gmail.com

--------------------------------------------------
     Emeritus Professor of Cryo-EM Data Processing
     Leiden University
     Mobile NL: +31(0)652736618 (ALWAYS ACTIVE SMS)
--------------------------------------------------
     Emeritus Professor of Structural Biology
     Imperial College London
     Faculty of Natural Sciences
     email: m.vanheel(A_T)imperial.ac.uk
--------------------------------------------------

I receive many emails per day and, although I try,
there is no guarantee that I will actually read each incoming email.