[3dem] [ccpem] Raw tif file micrographs and image format convertion
Marin van Heel
marin.vanheel at googlemail.com
Sun Jul 22 05:31:31 PDT 2018
Dear Yangqi Gu
Th EM2EM program will convert TIFF to any reasonable EM format. However
you do need to know what is in your TIFF variant. ( *bit 16 bit ...?)
The IMAGIC em2em program is available for free download from
ImageScience.de
Cheers
Marin
=======================================================
On 21/07/2018 15:46, Yangqi Gu wrote:
> Dear all,
> I am trying to process my EM micrographs. They are .tif images (Not a stacked movie, just a single image). And I tried using EMAN2 to convert them to .mrc using the command e2proc2d.py, however, it raised the following errors:
> raceback (most recent call last):
> File "/usr/local/bin/e2evalimage.py", line 1072, in <module>
> main()
> File "/usr/local/bin/e2evalimage.py", line 98, in main
> gui=GUIEvalImage(args,options.voltage,options.apix,options.cs,options.ac,options.box,options.usefoldername,options.constbfactor,options.astigmatism)
> File "/usr/local/bin/e2evalimage.py", line 166, in __init__
> n=EMUtil.get_image_count(i)
> File "/usr/local/lib/EMAN2db.py", line 539, in db_get_image_count
> raise Exception,fsp
> Exception: micrographs/WT_DV-001.tif
> I am wondering if anyone had any experience how to deal with this? Or can I just directly use .tif images?
> Best,
> Yangqi
>
> ########################################################################
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--
==============================================================
Prof Dr Ir Marin van Heel
Laboratório Nacional de Nanotecnologia - LNNano
CNPEM/LNNano, Campinas, Brazil
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email: marin.vanheel(A_T)gmail.com
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Emeritus Professor of Cryo-EM Data Processing
Leiden University
Mobile NL: +31(0)652736618 (ALWAYS ACTIVE SMS)
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Emeritus Professor of Structural Biology
Imperial College London
Faculty of Natural Sciences
email: m.vanheel(A_T)imperial.ac.uk
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