[3dem] [ccpem] removing outlier pixels

David A. Agard agard at msg.ucsf.edu
Fri Aug 3 11:14:25 PDT 2018


Outlier pixels of many kinds, not just bright pixels, are routinely detected and fixed by MontionCor2

David

David A. Agard
HHMI and Department of Biochemistry & Biophysics, UCSF

Sent from my iPhone. Please excuse brevity and any typos. 


> On Aug 3, 2018, at 10:21 AM, Marin van Heel <marin.vanheel at googlemail.com> wrote:
> 
> 
> Dear All,
> Our camera normalization program (IMAGIC-4D ) takes excellent care of such problems
> allowing you to perform a successful movie alignments on much smaller areas of the movies. See papers!
> Cheers
> Marin
> 
> https://www.nature.com/articles/srep10317
> http://journals.iucr.org/m/issues/2017/05/00/kf5002/
> 
>> On 03/08/2018 12:08, Noor Agip wrote:
>> Hi,
>> 
>> Can RELION-3 deal with outlier pixels (bright pixels) that may affect drift correction in the early parts of data processing? I know this can be done in cisTEM.
>> 
>> Best wishes,
>> 
>> Noor
>> ########################################################################
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> 
> 
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