[3dem] Gatan 910 multi specimen cryo transfer holder

Matthias Wolf matthias.wolf at oist.jp
Mon Oct 30 20:00:47 PDT 2017

Hi all,

We are considering the purchase of a Gatan 910 cryo transfer multi specimen side entry holder.

Does anyone have experience with this holder?
The specs with respect to stability are identical to the 626 (the alternative), but I don't know how it will perform in practice. It can take 3 grids.

The holder will be used for screening of a larger number of cryo samples. Cryo tomography is not a requirement. Due to possible contamination during unloading, the idea is not to reuse screened grids - once plunging conditions are established, a second grid frozen under identical conditions on a reproducible plunging bot will be used for data collection on a higher end cryo TEM with DED and autoloader. We hope to accelerate the cryo screening by loading 3 grids at once instead of one at a time and to reduce frosting and ice contamination with less cryo transfers. This step aims at offloading screening from the expensive high end tools and to maximize their beam time for productive data collection. The holder will also be used at room temperature for negative stained samples before cryo screening, both of which will act as triage steps for samples of insufficient quality.

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