[3dem] [TEM] amplitude contrast

[Dominika Elmlund]

Hi Sacha,
Philip did his PhD with R Schroeder.
Cheers,
Dominika

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*DOMINIKA ELMLUND PhD *
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simplecryoem.com

On 15 August 2017 at 22:29, Sacha De Carlo <sacha.decarlo at dectris.com>
wrote:

> ...thus my statement with a "may provide" instead of "will" (provide
> answers to...).
>
> Alright, let's try this again, forward 8 years (2000), Angert et al,
> zero-loss (no inelastic scattering) and of course in a practical sense the
> ice thickness and size of the aperture DO influence the contrast (for the
> same defocus or in-focus image), but I do not recall other papers where it
> was quantified methodically (thoroughly)...
>
> Cheers,
> Sacha
>
>
>
> On Tue, Aug 15, 2017 at 2:22 PM, Philip Koeck <Philip.Koeck at ki.se> wrote:
>
>> Hi Sacha.
>>
>>
>>
>> Thanks for the paper.
>>
>>
>>
>> I don’t think Wade says very much about the source of amplitude contrast.
>> He lists some possible explanations from other publications in section 2
>> of the paper,
>>
>> but to me it sounds a bit like nobody is really sure.
>> Therefore Wade and many others just continue with a phenomenological
>> description.
>>
>> I see many open questions:
>> If it’s due to scattering outside the aperture, shouldn’t it depend
>> strongly on the aperture size?
>> If it’s due to multiple scattering, it must depend strongly on specimen
>> thickness.
>>
>> If it’s due to inelastic scattering, why is it so weak (about 10%)?
>> Inelastic scattering has a larger cross section than elastic, I believe.
>>
>>
>>
>> All the best,
>>
>>
>>
>> Philip
>>
>>
>>
>>
>>
>> *Från:* Sacha De Carlo [mailto:sacha.decarlo at dectris.com]
>> *Skickat:* den 15 augusti 2017 13:11
>> *Till:* Philip Koeck
>> *Kopia:* 3dem at ncmir.ucsd.edu
>> *Ämne:* Re: [3dem] [TEM] amplitude contrast
>>
>>
>>
>> A good reference which may provide you with an answer... I am sure many
>> other people will provide more recent studies...
>>
>>
>>
>> Enjoy !
>>
>>
>>
>> Best,
>>
>> Sacha
>>
>>
>>
>>
>>
>>
>>
>> On Tue, Aug 15, 2017 at 12:55 PM, Philip Koeck <Philip.Koeck at ki.se>
>> wrote:
>>
>> Dear all.
>>
>>
>>
>> Is there any agreement on the cause of amplitude contrast when imaging
>> proteins in cryo-TEM?
>>
>>
>>
>> Is it the second order term in the expansion of a weak phase object or is
>> it due to inelastic scattering or the aperture or something else?
>>
>>
>>
>> Al the best.
>>
>>
>>
>> Philip
>>
>>
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>>
>>
>> --
>>
>> Sacha De Carlo, Ph.D.
>>
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> --
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> Sacha De Carlo, Ph.D.
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> 47 40 <+41%2079%20354%2047%2040> | Email: sacha.decarlo at dectris.com
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