[3dem] 3dem Digest, Vol 116, Issue 21

Craig Yoshioka yoshiokc at ohsu.edu
Fri Apr 28 14:22:18 PDT 2017


I’d like to add that you’ll know something has gone wrong with the heater if the charge seems to build much faster than you are expecting.


> On Apr 28, 2017, at 2:14 PM, Khoshouei, Maryam <maryamkh at biochem.mpg.de> wrote:
> 
> Dear Philip,
> 
> It is very important to have the heater on all the time. You wont get nice images without heating up the Volta phase plate.
> 
> Best,
> Maryam
> ---------------------------------------------
> Maryam Khoshouei, PhD student.
> Max Planck Institute of Biochemistry
> Department of Molecular Structural Biology
> Am Klopferspitz 18
> 82152 Martinsried
> Germany
> Tel: +49 (89) 8578-2651
> Fax: +49 (89) 8578-2641
> 
> 
> 
> ________________________________________
> From: 3dem [3dem-bounces at ncmir.ucsd.edu] on behalf of 3dem-request at ncmir.ucsd.edu [3dem-request at ncmir.ucsd.edu]
> Sent: Friday, April 28, 2017 9:00 PM
> To: 3dem at ncmir.ucsd.edu
> Subject: 3dem Digest, Vol 116, Issue 21
> 
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> Today's Topics:
> 
>   1. Re: Volta phase plate for G2 series Tecnai F30 (Philip Koeck)
>   2. Re: Volta phase plate for G2 series Tecnai F30 (Rado Danev)
> 
> 
> ----------------------------------------------------------------------
> 
> Message: 1
> Date: Fri, 28 Apr 2017 07:42:07 +0000
> From: Philip Koeck <Philip.Koeck at ki.se>
> To: Rado Danev <danev at biochem.mpg.de>, "3dem at ncmir.ucsd.edu"
>        <3dem at ncmir.ucsd.edu>
> Subject: Re: [3dem] Volta phase plate for G2 series Tecnai F30
> Message-ID:
>        <C8D24D7E19AEE543942A826EE910B1C2016B9084F7 at KIMSX02.user.ki.se>
> Content-Type: text/plain; charset="utf-8"
> 
> How important is the heater? Would it work without it?
> 
> Philip
> 
> -----Ursprungligt meddelande-----
> Fr?n: 3dem [mailto:3dem-bounces at ncmir.ucsd.edu] F?r Rado Danev
> Skickat: den 27 april 2017 14:34
> Till: 'Garry P Morgan'; 3dem at ncmir.ucsd.edu
> ?mne: Re: [3dem] Volta phase plate for G2 series Tecnai F30
> 
> Hi Garry,
> 
> Unfortunately I don't know of any alternative source for phase plate hardware. The phase plates themselves can be homemade - it's just a grid with ~10 nm amorphous carbon. But the aperture/phase plate holder is special with a built-in heater.
> 
> Cheers,
> 
> Rado
> 
> 
> -----Original Message-----
> From: 3dem [mailto:3dem-bounces at ncmir.ucsd.edu] On Behalf Of Garry P Morgan
> Sent: Monday, April 24, 2017 19:51
> To: 3dem at ncmir.ucsd.edu
> Subject: [3dem] Volta phase plate for G2 series Tecnai F30
> 
> Hello,
> i?m trying to get a quote for a Volta phase plate for a G2 Tecnai F30.  FEI has told me they don?t supply these.  can anyone tell me if there are other companies that do?
> thanks,
> Garry
> 
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> 3dem at ncmir.ucsd.edu
> https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem
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> ------------------------------
> 
> Message: 2
> Date: Fri, 28 Apr 2017 10:43:11 +0200
> From: Rado Danev <danev at biochem.mpg.de>
> To: 'Philip Koeck' <Philip.Koeck at ki.se>, <3dem at ncmir.ucsd.edu>
> Subject: Re: [3dem] Volta phase plate for G2 series Tecnai F30
> Message-ID: <06c701d2bffb$77bedfa0$673c9ee0$@biochem.mpg.de>
> Content-Type: text/plain; charset="UTF-8"
> 
> Hi Philip,
> 
> The heater is very important. Without it there will be beam-induced contamination and charging which will make the phase plate unusable.
> 
> Cheers,
> 
> Rado
> 
> 
> -----Original Message-----
> From: Philip Koeck [mailto:Philip.Koeck at ki.se]
> Sent: Friday, April 28, 2017 9:42
> To: Rado Danev <danev at biochem.mpg.de>; 3dem at ncmir.ucsd.edu
> Subject: SV: [3dem] Volta phase plate for G2 series Tecnai F30
> 
> How important is the heater? Would it work without it?
> 
> Philip
> 
> -----Ursprungligt meddelande-----
> Fr?n: 3dem [mailto:3dem-bounces at ncmir.ucsd.edu] F?r Rado Danev
> Skickat: den 27 april 2017 14:34
> Till: 'Garry P Morgan'; 3dem at ncmir.ucsd.edu
> ?mne: Re: [3dem] Volta phase plate for G2 series Tecnai F30
> 
> Hi Garry,
> 
> Unfortunately I don't know of any alternative source for phase plate hardware. The phase plates themselves can be homemade - it's just a grid with ~10 nm amorphous carbon. But the aperture/phase plate holder is special with a built-in heater.
> 
> Cheers,
> 
> Rado
> 
> 
> -----Original Message-----
> From: 3dem [mailto:3dem-bounces at ncmir.ucsd.edu] On Behalf Of Garry P Morgan
> Sent: Monday, April 24, 2017 19:51
> To: 3dem at ncmir.ucsd.edu
> Subject: [3dem] Volta phase plate for G2 series Tecnai F30
> 
> Hello,
> i?m trying to get a quote for a Volta phase plate for a G2 Tecnai F30.  FEI has told me they don?t supply these.  can anyone tell me if there are other companies that do?
> thanks,
> Garry
> 
> _______________________________________________
> 3dem mailing list
> 3dem at ncmir.ucsd.edu
> https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem
> 
> _______________________________________________
> 3dem mailing list
> 3dem at ncmir.ucsd.edu
> https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem
> 
> 
> 
> ------------------------------
> 
> Subject: Digest Footer
> 
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> 
> ------------------------------
> 
> End of 3dem Digest, Vol 116, Issue 21
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