[3dem] UA stock powder - age a factor?

[Regan M]

Dear Cornelia,

Post these issues regarding chemical fixation and classic EM in the
following group, http://www.microscopy.com/MicroscopyListserver
<http://www.microscopy.com/MicroscopyListserverOn-Line>
You are more likely to get right answers.

Greetings,
Johnson.

On Mon, Oct 3, 2016 at 2:02 PM, Cornelia Cazey <cornelia at strubi.ox.ac.uk>
wrote:

> Dear 3DEM list,
>
> I have up until now not had any major issues with making 2% UA negative
> stain. I am used to making stain and I used to be fairly consistent in
> getting good results. I am also making UFo, 0.75% which has no issues at
> all.
>
> In the past few months, however, the results of making UA stain have
> varied greatly and I am unable to figure out why. In order to find out,
> what might be the issue, I have tried a few variations in protocol (cooler
> water- longer mixing, different filter sizes)..
>
> I have just found out that the UA stock powder we use is roughly 14 years
> old... could this contribute to inconsistent results?
>
> Thanks for any help and best wishes,
>
> Cornelia Cazey
> TEM Technician
> OPIC - Oxford Particle Imaging Centre
> The Division of Structural Biology
> The Wellcome Trust Centre for Human Genetics
> University of Oxford
> Roosevelt Drive
> Oxford
> OX3 7BN
> Office:01865287843
> Mob:07981959647
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