[3dem] Graphene oxide grids for negative stain

Fri May 27 10:24:48 PDT 2016

Dear Sanchaita,

You might want to check a nice video made by Thomas Martin from the LMB in
Sjors Scheres group.
https://figshare.com/articles/Graphene_Oxide_Grid_Preparation/3178669

It's self-explanatory.

Cheers,
Ludo

On Fri, May 27, 2016 at 6:42 PM, Sanchaita Das <sanchaita06 at gmail.com>
wrote:

> Dear All,
> Has anyone tried Graphene oxide grids for negative staining of small
> proteins(<100KDa).
> If yes any tips and suggestions would be highly appreciated.
> Do you use normal Cu grids or holey carbon ones?
> Do you thin coat your grids with carbon or formvar before adding the
> Graphene?
> Regards,
> Sanchaita
>
> On Thu, May 26, 2016 at 3:00 PM, <3dem-request at ncmir.ucsd.edu> wrote:
>
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>> Today's Topics:
>>
>>    1. Re: Old camera and scanner to give out (kunihiro uryu)
>>    2. Research Scientist Position in the Simons Electron Microscopy
>>       Center at the New York Structural Biology Center (William Rice)
>>
>>
>> ----------------------------------------------------------------------
>>
>> Message: 1
>> Date: Thu, 26 May 2016 08:50:59 -0400
>> From: kunihiro uryu <kuryu at mail.rockefeller.edu>
>> To: Zongli Li <zongli.li at gmail.com>
>> Cc: 3DEM Mailing List <3dem at ncmir.ucsd.edu>
>> Subject: Re: [3dem] Old camera and scanner to give out
>> Message-ID:
>>         <
>> CAFK2HhbzsnV52OrN6wLK7Dv9YhDZKsptQvHz5OYUya+AHx1z1Q at mail.gmail.com>
>> Content-Type: text/plain; charset="utf-8"
>>
>> Hi Zongli,
>>
>> Thank you for letting me know. It was a great gift. I totally understand.
>>
>> Regards,
>> Hiro
>> ------
>> Kunihiro Uryu, Ph.D.,
>> Research Assistant Professor
>> Director of Electron Microscopy
>> Resource Center (EMRC)
>> RRB Rm120
>> The Rockefeller University
>> 1230 York Ave., Box 230
>> New York, NY 10065
>>
>> On Wed, May 25, 2016 at 7:28 PM, Zongli Li <zongli.li at gmail.com> wrote:
>>
>> > Hello everyone,
>> >
>> > Thanks a lot for your response. The two CCD cameras have been taken
>> > already. But
>> > two scanners are still available.
>> >
>> > Best regards,
>> >
>> > Zongli
>> >
>> > On Wed, May 25, 2016 at 5:08 PM, Zongli Li <zongli.li at gmail.com> wrote:
>> >
>> >> Sorry, I made a mistake when sending out previous email regarding the
>> CCD
>> >> camera:
>> >> Here is the correct message:
>> >>
>> >> 1. 1024x1024 Gatan MultiScan CCD, pixel size 24 um, model 794.
>> >> 2. 2048x2048 Gatan MegaScan CCD, pixel size 30 um (4k chips). Model
>> >> 794/20.
>> >> 3. Imaging plate scanner (DITABIS Micron scanner, Germany).
>> >> 4. SCAI scanner, used to digitize the negatives.
>> >>
>> >> Best regards,
>> >>
>> >> Zongli
>> >>
>> >> On Wed, May 25, 2016 at 4:09 PM, Zongli Li <zongli.li at gmail.com>
>> wrote:
>> >>
>> >>> Dear colleagues,
>> >>>
>> >>> We have following items we don't use anymore and would like to give
>> them
>> >>> out if someone can still use them:
>> >>>
>> >>> 1. 1024x1024 Gatan CCD camera, megascan, with 2k chips (pixel size 30
>> um)
>> >>> 2. Imaging plate scanner (DITABIS Micron scanner, Germany).
>> >>> 3. SCAI scanner, used to digitize the negatives.
>> >>>
>> >>> They are all in good working conditions.
>> >>>
>> >>> If interested, please contact me directly at
>> zongli_li at hms.harvard.edu.
>> >>> You will be responsible for arranging the shipment.
>> >>>
>> >>> Best regards,
>> >>>
>> >>> Zongli
>> >>>
>> >>
>> >>
>> >
>> > _______________________________________________
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>> Message: 2
>> Date: Thu, 26 May 2016 11:28:51 -0400
>> From: William Rice <rice at nysbc.org>
>> To: 3dem at ncmir.ucsd.edu, ccpem at jiscmail.ac.uk
>> Subject: [3dem] Research Scientist Position in the Simons Electron
>>         Microscopy Center at the New York Structural Biology Center
>> Message-ID: <57471633.8060803 at nysbc.org>
>> Content-Type: text/plain; charset="utf-8"; Format="flowed"
>>
>> Research Scientist Position in the Simons Electron Microscopy Center at
>> the New York Structural Biology Center
>>
>> The New York Structural Biology Center (NYSBC) seeks an experienced
>> electron microscopist to join the staff of the Simons Electron
>> Microscopy Center (http://semc.nysbc.org). The NYSBC is a shared center
>> that supports state-of-the-art research in EM, NMR, and X-ray
>> crystallography. The facilities include five transmission electron
>> microscopes (including 2xTitan Krios) and a dual-beam scanning electron
>> microscope (with a Quorum cryo stage), which support projects involving
>> electron tomography, single particle analysis and electron
>> crystallography of both stained and frozen-hydrated samples. Projects
>> focus on 3D reconstruction of biological assemblies ranging from the
>> atomic structure of membrane proteins, to the subunit organization in
>> macromolecular complexes and the cellular anatomy of developing
>> organisms, to 3D reconstructions of entire cells and their distributions
>> within their native tissue using the dual-beam microscope.
>>
>> To assist in these developments, NYSBC seeks an individual with
>> postdoctoral experience in biological electron microscopy and image
>> reconstruction. This individual will carry out experiments in support of
>> collaborative projects with affiliated investigators and will also have
>> opportunities to pursue independent research projects. The individual
>> should be capable of multitasking, should enjoy working with other
>> people, should have a good working knowledge of electron microscopes and
>> a strong research background. Good communication skills are essential.
>>
>> To apply, please send a c.v. and the names of three references to
>> emg at nysbc.org <mailto:emg at nysbc.org>.
>>
>> --
>> William Rice, Ph.D.
>> Senior Scientist
>> New York Structural Biology Center
>> 89 Convent Ave.
>> New York, NY 10027
>> (212) 939-0660 x344
>>
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>
>
>
> --
> "For one human being to love another! That is perhaps the most difficult
> of all tasks...The work for which all other work is but preparation"
> Rainer Maria Rilke
>
>
> *Sanchaita Das*
>
>
>
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