[3dem] Workshop announcement: Electron crystallography of 2D crystals of membrane proteins with 2DX, August 22-27, 2016

[Henning Stahlberg]

Hi,

Sorry, wrong dates:   August 22-27, 2016.  (Lectures August 23-26).

Henning.

Henning Stahlberg, PhD
Prof. for Structural Biology, C-CINA, Biozentrum, University Basel
Mattenstrasse 26 | D-BSSE | WRO-1058 | CH-4058 Basel | Switzerland
http://c-cina.org | Tel. +41-61-387 32 62



> On 11 May 2016, at 10:49, Henning Stahlberg <henning.stahlberg at unibas.ch> wrote:
> 
> Dear colleagues, 
> 
> We are happy to announce the fifth 2DX Workshop on Electron Crystallography of Membrane Proteins, which will take place at the University of Basel (Switzerland), on August 22-27, 2016. The workshop will provide hands-on training on practical aspects of 2D crystal data collection on a Titan Krios, and cover in depth the theoretical foundations and the practical image processing of 2D crystal cryo-EM images with the 2DX software package. No previous experience is required. 
> 
> Topics include: 
> Basics of 2D electron crystallography
> 	• Introduction to 2D electron crystallography
> 	• Cryo-electron microscopy sample preparation for 2D crystals of membrane proteins
> 	• Cryo-EM data collection of 2D crystals
> 	• Introduction to 2DX
> 	• Drift correction using ZORRO  
> Basic image processing with 2DX
> 	• Determination of the defocus, crystal tilt geometry, crystal lattice
> 	• Crystal Unbending - including movie-mode drift correction of individual unit cells in dose-fractionated image series
> 	• 2D merging and 3D merging with reconstruction via weighted back projection 
> 	• Automation of the processing pipeline
> Advanced topics in image processing with 2DX
> 	• Approaches for a partial reconstruction of amplitudes and phases inside of the missing cone
> 	• Processing of 2D crystal unit cells with RELION and FREALIGN, under exploitation of neighborhood correlation of certain image parameters
> 	• Advanced usage of the 2DX backend library
> 	• Preparation of final results with quantitative description of statistical values (e.g., resolution) for export to databases (EMDB).
> 
> Computing infrastructure will be provided in place and participants are welcome to bring their own data sets and/or computer (OSX or Linux). More information can be found at 
> http://www.2dx.unibas.ch/workshop/2016
> 
> For registration, please include information about your affiliation, and a short motivation statement (max 200 words).
> The registration fee is 250 CHF (travel and accommodation is not included), registration deadline is June 30th. As we can only provide a limited number of workstations, we typically accept participants on a "first come - first serve” basis. Please do not hesitate to contact us for any questions. Looking forward to welcoming you in Basel, 
> 
> Nikhil Biyani, 
> Ricardo Righetto,
> Robert McLeod,
> Henning Stahlberg.
> 
> Henning Stahlberg, PhD
> Prof. for Structural Biology, C-CINA, Biozentrum, University Basel
> Mattenstrasse 26 | D-BSSE | WRO-1058 | CH-4058 Basel | Switzerland
> http://c-cina.org | Tel. +41-61-387 32 62
>