[3dem] Image processing from the gold grids

[Xiaodi Yu]

Thank you so much Bob for sharing your experience.


Xiaodi

________________________________
From: Grassucci, Robert <rg2502 at cumc.columbia.edu>
Sent: Thursday, June 9, 2016 6:08:26 AM
To: Xiaodi Yu; 3dem at ncmir.ucsd.edu
Subject: RE: Image processing from the gold grids

Dear Xiaodi,
What you may be seeing is normalization/display  issue if the gold is a significant portion of your image.  Of course I am assuming that your ice is the same thickness in both types of grids.  In our hands the holey gold grids do seem to require a slightly longer blot time so that could also be the problem.  We have also noticed some gold particles on both the commercial and home-made holey gold grids but as long as it does not significantly alter the overall standard deviation it is not a problem .  I hope that helps you.
Best,
Bob


From: 3dem [mailto:3dem-bounces at ncmir.ucsd.edu] On Behalf Of Xiaodi Yu
Sent: Wednesday, June 08, 2016 11:27 PM
To: 3dem at ncmir.ucsd.edu
Subject: [3dem] Image processing from the gold grids




Dear All:



We have one dataset collected from the gold grids. We feel that the particle contrast from the gold grids is weaker comparing with the one from the regular carbon grids. We do find some gold particles in the images. So we wonder is it possible that these gold particles affect the image normalization/contrast? And is any prefilter or re-adjust the normalization of the images needed before we process the dataset? The last question is comparing with the carbon grids, do the gold grids prefer longer blotting time in order to get the thin ice?



This is the first time we collected the data from the gold grids. So any suggestion is welcome. Thank you in advanced.



Best ,



Xiaodi
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