[3dem] Image processing from the gold grids

Xiaodi Yu xiaodiyu at scripps.edu
Wed Jun 8 20:26:59 PDT 2016

Dear All:

We have one dataset collected from the gold grids. We feel that the particle contrast from the gold grids is weaker comparing with the one from the regular carbon grids. We do find some gold particles in the images. So we wonder is it possible that these gold particles affect the image normalization/contrast? And is any prefilter or re-adjust the normalization of the images needed before we process the dataset? The last question is comparing with the carbon grids, do the gold grids prefer longer blotting time in order to get the thin ice?

This is the first time we collected the data from the gold grids. So any suggestion is welcome. Thank you in advanced.

Best ,

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