[3dem] 3dem Ni-NTA nanogold and Negative stain EM

Sanchaita Das sanchaita06 at gmail.com
Fri Jul 15 09:37:09 PDT 2016


Dear All,
Would love to know if anyone has used Ni-NTA nano beads to do EM,
especially Negative stain EM. If yes may you share some tips about how to
make it work to get some decent data colelction and where may I purchase it
from.
Thanks in advance,
Sanchaita

On Wed, Jul 13, 2016 at 3:00 PM, <3dem-request at ncmir.ucsd.edu> wrote:

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> Today's Topics:
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>    1. Re: Correction: Tenure-track or tenured investigator      position
>       at NHLBI, NIH - Deadline Nov 20, 2016 (Jenny Hinshaw)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Tue, 12 Jul 2016 16:36:14 -0400
> From: Jenny Hinshaw <jennyh at helix.nih.gov>
> To: 3dem at ncmir.ucsd.edu
> Subject: Re: [3dem] Correction: Tenure-track or tenured investigator
>         position at NHLBI, NIH - Deadline Nov 20, 2016
> Message-ID: <9533785E-F415-4C2F-9533-AFCE72A9C204 at helix.nih.gov>
> Content-Type: text/plain; charset="utf-8"
>
> The deadline for the following position has been moved up to October 31,
> 2016.  I apologize for all the emails.  Feel free to contact me with any
> questions.
>
> Jenny
>
> > On Jul 12, 2016, at 4:14 PM, Jenny Hinshaw <jennyh at helix.nih.gov> wrote:
> >
> >
> > Investigator
> >
> > Cryo-Electron Microscopy
> >
> > Division of Intramural Research
> >
> > National Heart, Lung and Blood Institute, National Institutes of Health
> >
> > Department of Health and Human Services
> >
> >
> >
> > The Division of Intramural Research (DIR), National Heart, Lung, and
> Blood Institute (NHLBI), NIH is seeking an outstanding tenure-track or
> tenured investigator to initiate and direct an independent research program
> in basic science on the NIH campus in Bethesda.  This position is
> equivalent to Assistant Professor or Associate/Full Professor level (with
> the possibility of tenure at appointment) depending on qualifications and
> experience. We are specifically seeking applicants for the technical
> development and applications of Cryo-Electron Microscopy.
> >
> > The DIR environment provides the opportunity to perform creative and
> innovative science. The existing faculty is an outstanding group of
> internationally recognized biomedical researchers covering a wide range of
> basic and clinical research topics including biochemistry, biophysics,
> structural biology, cell biology, developmental biology, genetics,
> neuroscience, stem cell biology, and virology.  More detailed information
> about the Principal investigators at NHLBI DIR may be found at
> http://www.nhlbi.nih.gov/research/intramural/researchers/pi <
> http://www.nhlbi.nih.gov/research/intramural/researchers/pi>.
> >
> > In addition to a newly acquired cryo-EM infrastructure (an FEI Titan
> Krios with an energy filter and Gatan K2 direct detector is on order), the
> institute is committed to invest in new instrumentation, long-term
> operating costs and support personnel to create a state-of-the-art cryo-EM
> facility. A generous start-up package as well as a fully funded operating
> and personnel budget for the full duration of the appointment will be
> offered for the successful candidate?s individual research program.
> Furthermore, the investigator will have access to many outstanding research
> core facilities including optical and electron microscopy, biophysics,
> proteomics, DNA sequencing and genomics, transgenic, bioinformatics, flow
> cytometry, and pathology. More detailed information may be found at
> http://www.nhlbi.nih.gov/research/intramural/researchers/core <
> http://www.nhlbi.nih.gov/research/intramural/researchers/core>. These
> resources provide a unique research atmosphere unconstrained by traditional
>  support systems at academic or private research institutions.
> >
> > Applicants must have a Ph.D. (or equivalent) with significant experience
> in many aspects of cryo-electron microscopy and a record of independent
> scientific productivity as evidenced by citable publications. Excellent
> collaborative history is a plus.  Salary and appointment will be
> commensurate with qualifications and experience.  More detailed information
> about the NHLBI DIR may be found at
> http://www.nhlbi.nih.gov/research/intramural/ <
> http://www.nhlbi.nih.gov/research/intramural/>
> > Applicants may be US citizens, resident aliens or non-resident aliens
> with or eligible for a valid employment visa. Applications must be received
> by October 31, 2016. Please submit a curriculum vitae, brief statement of
> research interests and provide a list of three references in PDF or MS Word
> format only (no paper applications will be accepted) to:
> >
> > Robert S. Balaban, Ph.D., Scientific Director, NHLBI
> > c/o Dan Fogarty, IAMB General Administration Section Chief, NHLBI
> > nhlbi_recruit at mail.nih.gov <mailto:nhlbi_recruit at mail.nih.gov>
> >
> >
> > DHHS and NIH are Equal Opportunity Employers. Applications from women,
> minorities and persons with disabilities are strongly encouraged.
> >
> >
> > <NHLBI NIH Cryo-EM PI Search.pdf>
> > _______________________________________________
> > 3dem mailing list
> > 3dem at ncmir.ucsd.edu
> > https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem
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-- 
"For one human being to love another! That is perhaps the most difficult of
all tasks...The work for which all other work is but preparation"
Rainer Maria Rilke


*Sanchaita Das*
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