[3dem] Contamination guessing

[Petya Violinova Krasteva]

Hey Marcus,

We had a similar case working with bacterial samples (check out PMID:
25876066). In our case, it turned out to be AdhE spirosomes that both our
group and others thought to be something different to start with (RecA
nucleofilaments, type II pseudopili, etc.) but we managed to identify it
correctly. Plants seem to have a mitochondrial homolog that in some cases
could be abundant. Might be a long shot but worth to check it out for your
particular system.

In our PLoS Path paper we ended up purifying the filaments for
identification by MS. You should be able to reproduce the purification
protocol from the paper if you need to, but let me know if you need
details. Also, we bought an anti-AdhE antibody from a company called
Agrisera, that reacted with our homolog as well.

Good luck,

Petya

______________________

Petya V. Krasteva

Post-doctoral Research Associate
Institut Pasteur, France
Ph.D. Molecular and Cell Biology, 2011
Cornell University, NY

pvk2 at cornell.edu
+33 6 58 68 85 46

On Wed, Feb 24, 2016 at 3:50 PM, Fislage, Marcus <mf2944 at cumc.columbia.edu>
wrote:

> Dear all,
>
> I am working on a sample that is purified from plants and we are
> struggling with one particular contamination which:
> 1) is very abundant on our micrographs and
> 2) somehow not willing to be purified out.
>
> So far we have absolutely no idea on what it is and I thought that one of
> you might have seen something like this and knows what it is. Any good
> guess would be welcome.
> Attached you can find three 2D classes that are comprised of this thing.
> The box size is: 340 px = 428 A
> The particles mask is 360 A
> The length of this contamination can be incredibly long spanning distances
> of 30 nm to 400 nm.
> The diameter seems to be ~10 nm and the distance between the repeats is
> ~4-8 nm
>
> Thanks for any hints
> Cheers
> Marcus
>
>
> Marcus Fislage, PhD
>
> Howard Hughes Medical Institute (HHMI)
> Columbia University
> Department of Biochemistry and Biophysics
> Lab of Joachim Frank
> New York, NY
>
> email address: mf2944 at cumc.columbia.edu
> Phone: 212.305.9524
> Fax: 212.305.9500
>
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>
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