[3dem] Methods in Enzymology: Recent advances in cryoEM

[rac1 at mrc-lmb.cam.ac.uk]

Announcing that Methods in Enzymology volume 579 describing recent
advances in cryoEM will be published next week.  The chapters, listed below,
are already available on the Elsevier Methods of Enzymology website.

1. Direct electron detectors  (McMullan, Faruqi, Henderson, LMB)
2. Specimen behaviour in the electron beam (Glaeser, Berkeley)
3. Specimen preparation for high-resolution cryo-EM (Passmore & Russo, LMB)
4. Strategies for automated cryoEM data collection using direct detectors
(Cheng, Tan, Dandey, Potter, Carragher, NRAMM NY)
5. Processing of cryo-EM movie data (Ripstein, Rubinstein, Toronto)
6. Processing of structurally heterogeneous cryo-EM data in RELION
(Scheres, LMB)
7. Single particle refinement and variability analysis in EMAN2.1 (Ludtke,
Baylor)
8. FREALIGN: An exploratory tool for single particle cryo-EM (Grigorieff,
Janelia)
9. Testing the validity of single particle maps at low and high resolution
(Rosenthal, Crick London)
10. Tools for model building and optimization into near atomic resolution
cryo-electron microscopy density maps (DiMaio, Washington, Chiu, Baylor)
11. Refinement of atomic structures against cryo-EM maps (Murshudov, LMB)
12. Cryo-EM structure determination using segmented helical image
reconstruction (
Fromm & Sachse, EMBL)
13. Cryo-electron tomography and sub-tomogram averaging (Wan & Briggs, EMBL)
14. High resolution macromolecular structure determination by MicroED, a
cryo-EM
method (Rodriguez, UCLA-DOE, Gonen, Janelia)
15. Databases and archiving for cryoEM (Patwardhan, EBI Hinxton, Lawson,
Rutgers)

Tony Crowther, volume Editor, MRC LMB Cambridge