[3dem] Im6就會/`age distortion issues

Chen-Chou Wu wchenchou at gmail.com
Tue Jun 9 21:20:58 PDT 2015


還`阿
On Jun 4, 2015 1:04 AM, "William Nicolas" <wjnicolas at hotmail.com> wrote:

> Hello everyone,
>
> I am actually having trouble with coating my grids with gold particles to
> use as fiducial markers prior to tilt series acquisition.
>
> I usually use outdated secondary antibodies linked to 5nm gold particles
> but it went out. I thus bought a gold particle solution from BBI solution
> and can’t get the parameters straight.
> I tested different concentrations and different ways to put them on my
> grid and have found optimal settings.
>
> The problem is now that I want to use them on 200nm thick HM20 embedded
> sample slices which are deposited on a slot-grid coated with formvar, I
> have  distorted images on the camera BUT not on the fluorescent screen.
> The beads and image seems to be stretched in one direction and it is not
> astigmatism nor sample drifting. I thought of charges building up on the
> surface of the sample, distorting the electrons that are transmitted, maybe
> because of the BBI solutions liquid media…
>
> Do you have a solution to prevent charges from building up on the surface
> of my sample ?
>
> Thanks in advance.
>
> Regards,
>
> William Nicolas, PhD student, CNRS UMR 5200
>
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