[3dem] Advice needed on cryo Dewars

[Natesh Ramanathan]

Dear Karim, Mazdak and All,
        In continuation, I too have found World courier very reliable and
they can ship samples at desired temp. and they will give you a temperature
log
as well at various time intervals during the journey.
        Has anybody tried shipping cryo frozen grids on Dry ICE?
        I would think that shipping in Dry Shipper and Dry ICE would have
same effect.  May be Dry ICE is better?
        If you have any experience, please can you share your thoughts?
Best Regards,
Natesh

On 10 February 2015 at 13:18, Mazdak Radjainia <mazdak at mazdak.de> wrote:

> Dear Karim,
>
> I've send samples from New Zealand to Europe on three occasions (4
> dry-shippers in total), which is probably as far as anyone will ever ship
> grids. I had concerns but it works remarkably well. We used World Courier
> for shipping our CXR-100s because FedEx New Zealand wouldn't do it. Couple
> of lessons I learned along the way include:
>
> 1. Be careful with how you secure your grid boxes. I put my grids in
> circular boxes, which I locked with a handling rod like the one you find
> here (item 71165-50 at
> http://www.emsdiasum.com/microscopy/products/grids/accessories.aspx). All
> my grids fell out the first time I send samples to NeCEN. I think thermal
> expansion/contraction and vibrations during transport can cause the screws
> to become undone. Fortunately, we had enough uncontaminated areas left
> despite the grid sitting completely dry at the bottom of the Falcon tube!
> 2. Dry-shippers conform with IATA regulations and you can take them as
> carry-on but it's best to check with your airline and let them know.
> Singapore Airlines was fine with it.
> 3. Consider buying a hard case for your dry-shipper particularly if you
> are of Middle-Eastern appearance as much as I enjoyed having an entire
> subway train section during Munich's morning rush hour to myself. Might not
> work as well in let's say NYC and 'Fragile'/'This way up' stickers don't
> mean much to airport staff.
>
> Overall, frozen grids are a lot more resilient than I thought. The seal on
> one of my dry-shippers had been broken and someone had clearly looked
> inside at some stage in transit. Still no issue, which means that you can
> refrain from placing a mouse trap on top of your samples as tempting as it
> is.
>
> Anyway, good luck!
>
> cheers
> Mazdak
>
>
> On 4 February 2015 at 01:55, Karim benzerara <
> karim.benzerara at impmc.upmc.fr> wrote:
>
>> Hello
>>
>>
>>
>> We will perform x-ray microscopy observations on cryo-samples deposited
>> on TEM grids and prepared by plunge and freeze (something like 20-30
>> grids). We will prepare the samples in Paris and we will send them by FEDEX
>> to Berkeley. Would you recommend a specific DEWAR that will hold the trip
>> and keep our samples frozen for several days? What volume should I go for?
>> What brand? We are very new to this so apologize for the basic question.
>>
>>
>>
>> Thank you very much
>>
>>
>>
>> Karim Benzerara
>>
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>
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-- 
----------------------------------------------------------
"Live Simply and do Serious Things .. "
- Dorothy Mary Crowfoot Hodgkin OM, FRS

"In Science truth always wins"
- Max Ferdinand Perutz OM FRS
----------------------------------------------------------
Dr. Ramanathan Natesh
Ramalingaswami Fellow-DBT, Assistant Professor
Indian Institute of Science Education and Research Thiruvananthapuram
(IISER-TVM),
1st floor, Dept. of Computer Science & Engg. Building,
CET Campus, Engineering College P.O.,
Trivandrum,  695016, Kerala, India

natesh at iisertvm.ac.in
http://www.researcherid.com/rid/C-4488-2008
http://iisertvm.ac.in/~natesh
Ph. 0091- 471-2599403
Fax.0091-471-2597427
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