[3dem] Vitrobot tweezers

[Wim Hagen]

Joseph,

The price is pretty high because it is a precision made tool to make the Vitrobot reproducible with multiple tweezers. We had our workshop produce many cheap tweezers for all our users but unfortunately the mechanical alignment was all over the place, so I took all-but-one away and now our old Vitrobot Mk2 has somewhat good reproducibility again.

What our workshop did was basically what Bob described: just use the old tweezer as a model.

Once you start working with your new tweezers, the Vitrobot settings used so far may change significantly, you might have to adjust the blot-pad position/rotation and use different blot offset numbers.

This picture shows the alignment problem, “chaos, German style" :



Best regards,

Wim Hagen 
EMBL Heidelberg

> On Feb 4, 2015, at 1:23 AM, Grassucci, Robert <rg2502 at cumc.columbia.edu> wrote:
> 
> Joseph,
> I would suggest  you just remove the bent tweezers from the brass mounting plate and mill a standard set of tweezers to fit the mounting plate.  Just use the end of the old tweezers as the template.
> Bob
>  
>  
> From: 3dem-bounces at ncmir.ucsd.edu [mailto:3dem-bounces at ncmir.ucsd.edu] On Behalf Of Joseph Wang
> Sent: Tuesday, February 03, 2015 7:00 PM
> To: 3dem at ncmir.ucsd.edu
> Subject: [3dem] Vitrobot tweezers
>  
> Dear all,
>  
> I am thinking to make the customized tweezers for Vitrobot Mk III as the commercial ones are over my budget. Does anyone have a blueprint that can be kindly shared with?
>  
> Thank you and best regards,
> Joseph
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