[3dem] plunge with vitrobot
rg2502 at columbia.edu
Sun May 25 16:12:57 PDT 2014
Do you have any detergent or other material that would change the surface properties of the buffer? We see this behavior when working with membrane proteins that require them to stay suspended. I assume these are suspensions and not with thin carbon over the holey carbon. You wil notice that your protein is mostly in the thick areas. This is a good thing.
Sent from Bob's iPhone
> On May 25, 2014, at 5:29 PM, Xiaofeng Fu <xiaofeng at brandeis.edu> wrote:
> Dear all,
> Recently we had plunge problem by using vitrobot. Two images are attached: there is always a big droplet in the middle of square, and the ice is very thick at its neighboring area. We varied the GD from 10s to 40s at 24mA, but the grids look similar. The buffer doesn't contain any salt or sugar.
> Look forward to hearing any suggestions or experiences.
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