[3dem] etomo question dual axis tomogram generation

[cristina.suarez at bioquant.uni-heidelberg.de]

Dear colleagues,

I am trying to get a tomogram combination from two single tomograms  
using etomo, When I transfer the fiducials from other axis (tomogram  
"a" to "b") if I check the seed model the fiducials are wrong and  
etomo did not allow me to remove the wrong fiducials in order to put  
them in the righ position in the tomogram.
Any of us have an idea of the solution for this problem?
Aditionaly information, when I took the image stacks they were  
properly aligned after grid turning 90° and the single axis tomogram  
were generated without any problem.



Best regards


Cristina



Cristina Suárez García, PhD.
Electron Microscopy core facility & Dept. of Infectious diseases,
Heidelberg University,
Im Neuenheimer Feld 267, 69120
Heidelberg, Germany