[3dem] Summary posting: Cryo-genic shipping of cryo-EM grids

[Li, Huilin]

The attached word document contains eight responses to my request for people's experience on dry shipping the cryo-EM grids. Overall the community has a very positive experience: Dry shipping works. As you will see, the last response from Prof. James Conway in the University of Pittsburg is perhaps most useful, as it contains several photos and very practical instructions and tips.

Thanks.

Huilin Li

________________________________
From: 3dem-bounces at ncmir.ucsd.edu [3dem-bounces at ncmir.ucsd.edu] on behalf of Li, Huilin [hli at bnl.gov]
Sent: Sunday, March 02, 2014 8:01 PM
To: 3dem at ncmir.ucsd.edu
Subject: [3dem] Cryo-genic shipping of cryo-EM grids

We are considering to FedEx several cryo-EM grids with a Dry-shipping dewar. According to the guideline for cryogenic shipping of frozen protein crystals,

http://smb.slac.stanford.edu/facilities/hardware/cryotools/shipping-dewar-testing.html

a quality dewar would keep the content cold (below -180 degree Celsius) for a week. But we have never done this before, and are worried if the cryo-grids would be contaminated even if the temperature is maintained low.

We would appreciate any experience you may be able to share with us.

Huilin Li


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