[3dem] 答复: Cryo-genic shipping of cryo-EM grids

[Hongwei Wang]

Huilin,

 

We have done the same many times shipping samples from US to Japan and
China. Grids are maintained good in the dry shipper. Just make sure that the
dry shipper is fully cooled by filling it up several times before the
shipping. On the day that the Fedex pick up the dewar, simply empty the
liquid nitrogen (I normally leave a little liquid inside) and seal the cap
with a plastic string.

 

Best regards,

Hongwei

 

发件人: 3dem-bounces at ncmir.ucsd.edu [mailto:3dem-bounces at ncmir.ucsd.edu] 代
表 Zhang, Peijun
发送时间: 2014年3月3日 11:34
收件人: Li, Huilin; 3dem at ncmir.ucsd.edu
主题: Re: [3dem] Cryo-genic shipping of cryo-EM grids

 

Hi Huilin,

 

We have been using dry-shipper to send cryo-EM grids many times to a number
of places, California and even Netherland, and we have not yet experienced
any problem with dry-shipper. It works quite well.

 

Peijun

 

From: 3dem-bounces at ncmir.ucsd.edu [mailto:3dem-bounces at ncmir.ucsd.edu] On
Behalf Of Li, Huilin
Sent: Sunday, March 02, 2014 8:01 PM
To: 3dem at ncmir.ucsd.edu
Subject: [3dem] Cryo-genic shipping of cryo-EM grids

 

We are considering to FedEx several cryo-EM grids with a Dry-shipping dewar.
According to the guideline for cryogenic shipping of frozen protein
crystals, 

 

http://smb.slac.stanford.edu/facilities/hardware/cryotools/shipping-dewar-te
sting.html

 

a quality dewar would keep the content cold (below -180 degree Celsius) for
a week. But we have never done this before, and are worried if the
cryo-grids would be contaminated even if the temperature is maintained low.

 

We would appreciate any experience you may be able to share with us.

 

Huilin Li

 

 

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