[3dem] Company Recommendations for Producing Antibodies Raised Against Peptides for IEM

Mannella, Carmen (HEALTH) carmen.mannella at health.ny.gov
Fri Jun 27 09:08:27 PDT 2014


I can only provide caveat that anti peptide antibodies in our hands generally worked well for western blot applications, ok for suspension work, but poorly for immunoEM.  A lot depends on how rationally you can select optimum linear epitopes

Carmen Mannella

Sent from my iPhone

> On Jun 27, 2014, at 11:05 AM, "Evan Rossignol" <edr5 at bu.edu> wrote:
> Hi all,
> We are starting an ImmunoEM project that requires primary antibodies made against synthetic peptides, a subject I know little about.  We are wondering if anyone had any wisdom or experience with companies to go with or to avoid.
> Optimally they'd produce the peptide and raise the serum and/or monoclonal antibodies.
> Thanks!
> -Evan Rossignol
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