[3dem] amplitude contrast valuesHi,

[Javier Vargas]

Dear David,

I take the opportunity of your message to advice about a new CTF
determination approach which does not require the amplitude contrast as
input.

You can find it at:

http://www.ncbi.nlm.nih.gov/pubmed/23261401

Best Regards,

Javier

Date: Mon, 21 Jul 2014 13:02:23 -0400
> From: David Gene Morgan <dagmorga at indiana.edu>
> To: 3dem at ncmir.ucsd.edu
> Subject: [3dem] amplitude contrast valuesHi,
> Message-ID: <53CD479F.9030306 at indiana.edu>
> Content-Type: text/plain; charset=ISO-8859-1; format=flowed
>
> Hi,
>
>         I'm trying to do some automated defocus determination (initially
> trying
> Niko's ctffind3 program) and would like some input on appropriate
> amplitude contrast values for various types of samples.  I know that ice
> embedded images usually are said to have an amplitude contrast of 0.07
> to 0.1.  I also know that with protein/nucleic acid complexes in ice,
> amplitude contrast may need to be set a bit higher (say to as much as
> 0.15 for some data I worked on awhile ago).
>
>         But I don't have any sort of feeling for appropriate values of
> amp;itude contrast for different sorts of negative stains (either cryo
> negative stain or standard negative stain).  It should be "high," but I
> don't know how high might be appropriate.
>
>         If anyone has either some practical or theoretical insight into
> this,
> I'd appreciate some suggestions.  Also, does anyone know about an
> appropriate amplitude contrast for a standard Au/Pd waffle grid?
>
>         Thanks in advance for all your help.
>
> --
>                   David Gene Morgan
>               Electron Microscopy Center
>                    047D Simon Hall
>                    IU Bloomington
>                 812 856 1457 (office)
>                 812 856 3221 (3200)
>              http://bio.indiana.edu/~cryo
>
>
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> End of 3dem Digest, Vol 83, Issue 11
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