[3dem] amplitude contrast valuesHi,

[Sacha De Carlo]

Hi David,

0.2 - 0.3 is not unreasonable for (cryo) neg.st. I had the values written in one of my reviews, but I don’t remember which one :)

Cheers,
Sacha


On Jul 21, 2014, at 7:02 PM, David Gene Morgan <dagmorga at indiana.edu> wrote:

> Hi,
> 
> 	I'm trying to do some automated defocus determination (initially trying Niko's ctffind3 program) and would like some input on appropriate amplitude contrast values for various types of samples.  I know that ice embedded images usually are said to have an amplitude contrast of 0.07 to 0.1.  I also know that with protein/nucleic acid complexes in ice, amplitude contrast may need to be set a bit higher (say to as much as 0.15 for some data I worked on awhile ago).
> 
> 	But I don't have any sort of feeling for appropriate values of amp;itude contrast for different sorts of negative stains (either cryo negative stain or standard negative stain).  It should be "high," but I don't know how high might be appropriate.
> 
> 	If anyone has either some practical or theoretical insight into this, I'd appreciate some suggestions.  Also, does anyone know about an appropriate amplitude contrast for a standard Au/Pd waffle grid?
> 
> 	Thanks in advance for all your help.
> 
> -- 
>                 David Gene Morgan
>             Electron Microscopy Center
>                  047D Simon Hall
>                  IU Bloomington
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