[3dem] Protochips C-flat ice thickness

[Daniel Nemecek]

Hi Reza,

I had no problem to freeze such big particles (200 nm) on lacey-carbon 
grids using a Vitrobot. Ice thickness was about 220 nm as determined by 
tomography. I don't see why it shouldn't work with C-flats as well.

Best,
Daniel


On 07/10/2014 03:10 AM, rkhayat at ccny.cuny.edu wrote:
> Hi,
>
> I have some large particles (200-300nm) that I'd like to
> freeze for cryo studies. Has anyone had any luck freezing
> particles this size with Protochips C-flat grids? Thanks.
>
> Best wishes,
> Reza
>
> Reza Khayat, PhD
> Assistant Professor
> The City College of New York
> Department of Chemistry, MR-1135
> 160 Convent Avenue
> New York, NY  10031
> Tel. (212) 650-6070
> www.khayatlab.org
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-- 
Daniel Nemecek, PhD
CryoEM Research Group
CEITEC / Masaryk University
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62500 Brno, Czech Republic
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