[3dem] cleaning grids before freezing

Bill & Sue Tivol wtivol at sbcglobal.net
Wed Aug 13 10:05:56 PDT 2014

On Aug 5, 2014, at 9:27 AM, David Gene Morgan wrote:

> Hi,
> 	What are people doing to treat/clean Quantifoil and C-Flat grids  
> before freezing?
> -- 
>                 David Gene Morgan
>             Electron Microscopy Center
>                  047D Simon Hall
>                  IU Bloomington
>               812 856 1457 (office)
>               812 856 3221 (3200)
>            http://bio.indiana.edu/~cryo
> _______________________________________________
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> 3dem at ncmir.ucsd.edu
> https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem

Dear David,
	I always glow-discharge for ~1 min and put ~3 uL colloidal gold on  
the grid, then use just after it dries.  If you do not want the gold  
on your grid, omit that step.  When I put the specimen on the grid, I  
check to see if there is a meniscus on both sides.  If so, then the  
specimen has wet the grid and I am confidant that the plunging will  
give me a good distribution with specimen in all the holes.

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