[3dem] cleaning grids before freezing
Bill & Sue Tivol
wtivol at sbcglobal.net
Wed Aug 13 10:05:56 PDT 2014
On Aug 5, 2014, at 9:27 AM, David Gene Morgan wrote:
> What are people doing to treat/clean Quantifoil and C-Flat grids
> before freezing?
> David Gene Morgan
> Electron Microscopy Center
> 047D Simon Hall
> IU Bloomington
> 812 856 1457 (office)
> 812 856 3221 (3200)
> 3dem mailing list
> 3dem at ncmir.ucsd.edu
I always glow-discharge for ~1 min and put ~3 uL colloidal gold on
the grid, then use just after it dries. If you do not want the gold
on your grid, omit that step. When I put the specimen on the grid, I
check to see if there is a meniscus on both sides. If so, then the
specimen has wet the grid and I am confidant that the plunging will
give me a good distribution with specimen in all the holes.
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