[3dem] "leopard skin" ice

[Jacob Brink]

Hi Paul and Frank,

We've also seen this type of ice in Flu particle preps at NIBSC during the cryo-EM course, but we'd see it in the middle of the ice, away from particles (see below). The ethane tank had not been moved. So, we never really could attribute it to anything unless the ice would get really thin? Open air plunger (!) and imaged in a 2100-LaB6 TT.

Jaap



On Nov 21, 2013, at 2:36 PM, Paul Chipman <pchipman at ufl.edu> wrote:

> Hi Frank,
> 
> At Purdue we called this "alligator ice" and would see it from time to time, occasionally when an ethane tank was nearing empty.  As such we attributed to impurities that impeded freezing.  It could also be just something in the buffer or sample that alters the freezing.  The recent image below displays this type of ice but only near the viral particles.  Most of the ice was great except in areas of high particle concentration.  When I told the student the name we gave this type of ice, I got a laugh.
> 
> Go Gators! (Florida)
> 
> Cheers,
> Paul 
> 
> 
> On Wed, Nov 20, 2013 at 1:54 AM, <frankpolzer at physik.hu-berlin.de> wrote:
> Dear all,
> 
> During the last weeks, I frequently observe what I believe is so-called
> leopard skin ice in my vitrified samples (plunge frozen in ethane using
> Mark IV Vitrobot).
> 
> What again is the reason for this type of ice and how can this be avoided?
> 
> Thanks for your help in advance,
> 
> Frank
> 
> 
> Dr. Frank Polzer
> 
> TEM Group
> Insitute of Physics
> Humboldt Universität zu Berlin
> Newtonstraße 15
> 12489 - Berlin
> Tel.: +49 30 2093-4995 (office)
> Tel.: +49 30 2093-7829 (TEM)
> Fax: +49 30 2093-7886
> 
> 
> _______________________________________________
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> 
> 
> 
> -- 
> Paul Chipman
> Assistant Director of Research
> Biochemistry and Molecular Biology
> University of Florida
> 352-294-1790
> 
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