[3dem] protein localization

Kong, Xiangpeng xiangpeng.kong at med.nyu.edu
Mon Feb 11 06:59:49 PST 2013


I don't remember it was returned again. I'll look into it again. However,
we are not transfer the animal into the OPLH building for keeping their,
we just want to take them out and sacrifice them for tissue/blood. Would
that be okay?

On 2/11/13 9:22 AM, "Kimberley Cheng" <Kimberley.Cheng at vironova.com> wrote:

>Thank you everyone for your quick response! I really appreciate the help.
>
>All the best,
>Kimberley 
>
>
>
>Kimberley Cheng, Ph.D.
>Senior Scientist, Electron microscopy
>
>Vironova AB (publ)
>Gävlegatan 22
>SE-113 30 Stockholm
>SWEDEN
>
>Tel: +46-(0)-8-702 67 90
>Mobile: +46-(0)76-147 29 07
>Fax: +46-(0)-8-702 67 91
>E-mail: kimberley.cheng at vironova.com
>Web: www.vironova.com
>
>
>-----Original Message-----
>From: 3dem-bounces at ncmir.ucsd.edu [mailto:3dem-bounces at ncmir.ucsd.edu] On
>Behalf Of rkhayat at ccny.cuny.edu
>Sent: den 8 februari 2013 17:41
>To: Kimberley Cheng; 3dem at ncmir.ucsd.edu
>Subject: Re: [3dem] protein localization
>
>Hi,
>
>I may be suggesting something you've already tried, but why not try some
>of the traditional membrane protein proteolysis experiments?
>     1. Proteolyse the protein with a protease to make sure it can be
>digested
>     2. Decorate the liposome with the protein
>     3. Purify the protein decorated liposome from free protien
>     4. Treat the decorated liposome with protease
>     5. Test to see if the protease can digest in the presence of
>liposomes by running the same proteolysis experiment in the presence of
>the liposomes and a digestible protein that is not incorporated into the
>liposome
>
>Reza Khayat, PhD
>Assistant Professor
>The City College of New York
>160 Convent Avenue
>Department of Chemistry
>MR-1335
>Tel. (212) 650-6070
>
>
>---- Original message ----
>>Date: Fri, 8 Feb 2013 15:56:06 +0000
>>From: 3dem-bounces at ncmir.ucsd.edu (on behalf of Kimberley Cheng
>><Kimberley.Cheng at ki.se>)
>>Subject: [3dem] protein localization
>>To: "3dem at ncmir.ucsd.edu" <3dem at ncmir.ucsd.edu>
>>
>>   Hi,
>>
>>   My colleague is working with protein-liposome
>>   complexes. She thinks the proteins are either
>>   incorporated in the liposomal membrane, or attached
>>   to the surface of the liposomes. Is there any EM
>>   method, or some other technique, that can be used to
>>   assess where the proteins are located? Someone
>>   suggested immunogold-labelling. Any thought about
>>   this?
>>
>>   All the best,
>>
>>   Kimberley
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