[3dem] Gatan cryo-holder question

[Ruben Diaz]

Hi David,

I really have no idea why this happens (probably Maxwell's demon doing one of its tricks), but I also have seen this problem with the tomography cryoholder. The way I got rid of this problem is by putting a tiny dab of formvar in chloroform (a 1% solution works fine) on the head of the screw. The tiny bit that stays at the tip of a toothpick is enough, as it runs into the screw and most of its surfaces, fixing them more or less securely.

Happy holidays to you too!

Ruben.

*******************************
Ruben Diaz-Avalos, Ph.D.
Department of Molecular and Cellular Biology,
Briggs Hall,
University of California, Davis,
1 Shields Avenue,
Davis, CA 95616
Phone: (530)752-8282
Cell:      (917)515-6353
*******************************



On Dec 15, 2013, at 11:37 AM, David Gene Morgan <dagmorga at indiana.edu> wrote:

> Hi,
> 
> 	In the last six months, we have lost a screw from the tip of both our 
> Gatan 626 and Gatn 914 cryo-holders.  Has anyone else had similar 
> problems, and does anyone have suggestions for why this may be 
> happening?  Thanks, and Happy Holidays!
> 
> -- 
>                  David Gene Morgan
>              Electron Microscopy Center
>                   320C Simon Hall
>                   IU Bloomington
>                812 856 1457 (office)
>                812 856 3221 (3200)
>             http://bio.indiana.edu/~cryo
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> 3dem mailing list
> 3dem at ncmir.ucsd.edu
> https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem

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