[3dem] Don't blame your thermometer...

[Marin van Heel]

Thought of the day:


If you overheat your house, do you blame your thermometer?
It is rather your own responsibly to change your thermostat settings!

If you over-fit your 3D reconstruction, do you blame the FSC resolution 
measure?

It is rather your own responsibility to avoid overfitting by reference 
bias.
There is no such thing as a biased FSC (Fourier Shell Correlation). 
Neither is there a "gold standard" FSC!
Yes, you CAN do deviously biased data processing to try to prove you are 
better than everybody else.
But what ever you do, please don't blame your thermometer.

Cheers,
Marin

-- 
================================================================

     Marin van Heel

     Professor of Cryo-EM Data Processing

     Leiden University
     NeCEN Building Room 05.27
     Einsteinweg 55
     2333 CC Leiden
     The Netherlands

     Skype:    Marin.van.Heel
     email:  marin.vanheel(A_T)gmail.com
     and:    mvh.office(A_T)gmail.com

----------------------------------------------
     and:

     Professor of Structural Biology

     Imperial College London
     Faculty of Natural Sciences
     Biochemistry Building (Room 512)
     South Kensington Campus
     London SW7 2AZ,  UK
     email:  m.vanheel(A_T)ic.ac.uk

----------------------------------------------
     Currently visiting Professor at:

     Laboratório Nacional de Nanotecnologia - LNNano
     CNPEM/ABTLuS, Campinas, Brazil

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