[3dem] Lack of Lateral Views

[Veer Bhatt]

Dear all,

I am working on a multimeric membrane protein that has a strong  
tendency to form rings. The top views of these rings can be visualized  
rather easily using negative-staining EM. However, I am unable to find  
ANY lateral views. Could someone suggest a solution? CryoEM is not  
feasible for me at the moment.

I use Uranyl Acetate for staining over '300 mesh' carbon (pre)coated  
copper grids.

Thanks a lot,
Veer.

Veer Sandeep Bhatt

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