[3dem] Sunday short course at M&M meeting 2012

[Michael Radermacher]

Dear All,

I would like to make you aware of the Sunday (July. 29, 8:30 AM-5 PM)
short course on 3D electron microscopy held at the Microscopy and
Microanalysis meeting Jul 29-Aug. 2 in Phoenix, AZ. This course should not
only be interesting to biologists, but also for people in the material
sciences field interested in 3D electron microscopy. Registration for
this course is still open
(http://www.microscopy.org/MandM/2012/register.cfm, extra item).
Description below.

X14: 3D Electron Microscopy of Macromolecular Assemblies
Teresa Ruiz, Michael Radermacher, Ed Morris

This short course will provide a comprehensive description of the
methods used for 3D structure determination of macromolecular
complexes from electron micrographs. First specimen preparation
techniques for single particles (deep stain, vitreous ice), will be
presented and the selection of imaging conditions including low-dose
imaging. This will be followed by a detailed explanation of image
processing techniques with special emphasis on the random conical
reconstruction technique. In the last part structure interpretation
and docking of X-ray structures to 3D EM densities will be demonstrated.

-- 
Michael Radermacher, Ph.D., Prof.
University of Vermont
Dept. Molecular Physiology and Biophysics
HSRF 120 / 149 Beaumont Ave
Burlington, VT 05405
USA