[3dem] Re: 64bit MRC conversion

[Steven Ludtke]

Ok, not to beat a dead horse, but Mario just pointed out that we did, indeed fix this in EMAN1 already, but
as we don't do new releases for EMAN1 any more, you will need to get a 'nightly snapshot' version of EMAN1
to get support for FEI MRC files. With a snapshot you should be able to open the images directly in boxer. 
----------------------------------------------------------------------------
Steven Ludtke, Ph.D.
Associate Professor, Dept. of Biochemistry and Mol. Biol.      Those who do
Co-Director National Center For Macromolecular Imaging	           ARE
Baylor College of Medicine                                     The converse
sludtke at bcm.edu  -or-  stevel at alumni.caltech.edu               also applies
http://ncmi.bcm.edu/~stevel

On Jan 5, 2012, at 1:34 PM, Mario J. Borgnia wrote:

> Steve,
> 
> I believe that one of the eman.daily includes a version of boxer that is able to read the newer FEI mrc files. 
> 
> 
> Mario
> 
> On 01/05/2012 02:14 PM, Steven Ludtke wrote:
>> 
>> Ahh. I understand. Eman2 will read the Fei mrc files, but I don't believe we ever back ported to eman1. They are not generally readable by software that handles generic MRC files. With eman2:
>> 
>> e2proc2d.py file.mrc file.hdf 
>> 
>> should produce something boxer can read, without losing the extra metadata. I suppose we should update the reader for eman1 as well. It's very frustrating when companies take it upon themselves to change established standards, even if only slightly.
>> 
>> Sent from my iPhone
>> 
>> On Jan 5, 2012, at 10:41 AM, toropova <toropova at fas.harvard.edu> wrote:
>> 
>>> Dear all, 
>>> 
>>> Thanks so much for all the inputs on my query.
>>> 
>>> It seems the files I have may not be 64-bit MRCs, we got them from FEI so I'm going to e-mail them to find out exactly what we have but while EM2EM, ImageJ and proc2d could not read them, bimg from Bsoft could (thanks Terje Dockland).
>>> 
>>> All the best, 
>>> Kat
>>>   
>>> Katerina Toropova
>>> Postdoctoral Research Fellow (Leschziner lab)
>>> Dept. of Molecular and Cellular Biology
>>> Harvard University
>>> 305 NWB, 52 Oxford St.
>>> Cambridge, MA 02138
>>> 
>>> 
>>> 
>>> On 5 Jan 2012, at 11:28, Katerina Toropova wrote:
>>> 
>>>> Dear EM community, 
>>>> 
>>>> Does anyone know how to convert 64-bit MRC image files to a format that Boxer can read, e.g. 8/16-bit TIFF?
>>>> 
>>>> Many thanks, 
>>>> Kat
>>>>   
>>>> Katerina Toropova
>>>> Postdoctoral Researcher 
>>>> Dept. of Molecular and Cellular Biology
>>>> Harvard University
>>>> 305 NWB, 52 Oxford St.
>>>> Cambridge, MA 02138
>>>> 
>>>> 
>>>> 
>>> 
>>> _______________________________________________
>>> 3dem mailing list
>>> 3dem at ncmir.ucsd.edu
>>> https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem
>> 
>> 
>> _______________________________________________
>> 3dem mailing list
>> 3dem at ncmir.ucsd.edu
>> https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem
> 
> 
> -- 
> +---------------------------------+
> | Mario J. Borgnia, Ph. D.        |
> |                                 |
> | Lab of Cell Biology             |
> | NIH NCI/CCR                     |
> | 50 South Drive Rm 4306 MSC 8008 |
> | Bethesda MD 20892-8008          |
> |                                 |
> | Tel: (301) 594 0563             |
> | mborgnia at nih.gov                |
> +---------------------------------+

-------------- next part --------------
An HTML attachment was scrubbed...
URL: https://mail.ncmir.ucsd.edu/mailman/private/3dem/attachments/20120105/ef333090/attachment.html