[3dem] Image file format of Eagle 4K CCD and conversion

[Marin van Heel]

Dear Homin Kim,

You can always convert to MRC or  IMAGIC format using the (free 
download) EM2EM program starting from 16 bit TIFF.
If I understood things correctly, the newest version of EM2EM should 
also be capable of reading of  *.SER directly (created by TIA) and 
convert it to all other popular EM formats including IMAGIC and MRC. If 
your version does not yet have that option you can use the OFFSET option 
in EM2EM to jump over the 126 byte header just as  suggested by Sacha. 
Conversion of the raw binary data should also be possible.  If you 
experience any problems during the conversion please contact Ralf!

Cheers

Marin van Heel

=============================

On 23-Aug-12 11:28 AM, hohu78 at gmail.com wrote:
> Dear all,
> I have installed the Eagle 4K CCD camera from FEI on Tecnai G2 spirit.
> Because I only have experienced Gatan camera, I'm struggling to figure 
> out this camera and software.
> Questions are
> 1. How to convert emi file format which is the basic option in eagle 
> camera to MRC or IMAGIC format?
>     Is there any available free software?
> 2. Using export option in software, I can also save image as several 
> formats,
>     like Tif (full resolution), Bmp(full resolution), JPG (full 
> resolution), Raw binary (.bin), True image (.ebn).
>     Which file format is the best one for single particle analysis 
> without losing the information of image?
> Anyone who have experiences, give me valuable suggestions, please.
> Many thanks,
> Best regards,
> Homin Kim
> Homin Kim, Ph D.
> Korea Advanced Institute of Science and Technology (KAIST)
> Graduate School of Medical Science and Engineering
> BioMedical Research Center(E7) Room 2102, KAIST
> 291 Daehak-ro, Yuseong-gu
> Daejeon, 305-701, Republic of Korea
> Phone: +82-10-7184-5521(Cellphone), +82-42-350-4244 (Office)
> FAX : +82-42-350-4240
> Email: hm_kim at kaist.ac.kr
>
>
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-- 
================================================================

     Marin van Heel


     Professor of Cryo-EM Data Processing

     Leiden University
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     and:

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