[3dem] Re: 3dem Digest, Vol 49, Issue 6
DocDave50 at aol.com
DocDave50 at aol.com
Mon Sep 12 22:22:44 PDT 2011
Peter,
While I don't have specific experience with the Edwards Turbo Station, I
do have experience with the Gatan Pumping Station and a Pumping Station I'd
built while at Yale which has a rotary pump drafted Turbo. In my system, a
vacuum of 10e-7 Torr can be achieved in 30 minutes where the Gatan Pumping
Station yields, at best, 0.5e-6 Torr over a 24 hour pumping cycle. The
advantage of the Turbo Pumping stations...I believe that the Edwards are
usually 'drafted' turbo systems...is a much faster dewar turn-around and given
the higher vacuums being pulled, I have found much greater holder stability
over much longer times of sample viewing. If there is any relationship
between the Edward vacuum pumping systems in their Evaporator Systems, I know
these can also be run with liquid nitrogen cooling which provides yet
deeper vacuum conditions. I don't know if my experience is unique but it says
something very positive about the higher level turbo pumping systems in
facilitating better holder stability over time.
David W. Chester, Ph.D.
Department of Biochemistry
Center for Structural Biology
Flowers Building
Imperial College of England
Exhibition Road
South Kensington, London, UK. SW7 2AZ
_d.chester at Imperial.ac.uk_ (mailto:d.chester at Imperial.ac.uk)
In a message dated 9/12/2011 3:00:05 P.M. Eastern Daylight Time,
3dem-request at ncmir.ucsd.edu writes:
Dear 3DEM-ers,
Does anyone have experience with evacuating a (Gatan) cryo-holder with an
Edwards Turbo station (TS75D) ?
vriendelijke groeten,
Peter
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