[3dem] problem on EMAN2 programme e2proc3d

[Steven Ludtke]

Hi CJ. I would suggest using the EMAN mailing list/group for questions like this:
https://groups.google.com/forum/?hl=en#!forum/eman2

Regardless, there are a couple of issues here:

1) You are not using EMAN2's e2proc3d.py, but EMAN1's proc3d command. EMAN1's windows support is much less
complete than EMAN2's. 

2) My suspicion is that you ran into a memory limitation under 32 bit windows
(though 680^3*4 is only 1.2 GB, so this seems a bit strange). Anyway, it would have helped if you
provided the actual error message you got.

I would suggest trying EMAN2, it is much more stable on windows, and if you do get an error it will be
much easier to interpret.

----------------------------------------------------------------------------
Steven Ludtke, Ph.D.
Associate Professor
Co-Director National Center For Macromolecular Imaging
Dept of Biochemistry and Mol. Biol.
Baylor College of Medicine
sludtke at bcm.edu
stevel at alumni.caltech.edu




On Sep 8, 2011, at 2:58 AM, C.J. wrote:

> Dear all,
> 
> When I ran the e2proc3d.py at windows2008server platform and happened
> to a error as following.
> -------------------------------------------
> E:\>proc3d tmv.map tmv2.mrc scale=2 clip=400,400,400
> EMAN 1.9 ($Date: 2009/02/18 05:12:22 $).
> Run 'proc3d help' for detailed help.
> Original image : 200x200x200 Mean=0.551 Sigma=5.74 Min=-26.3 Max=46.9
>      Sampling : 1.24 A/pixel   Origin = 0,0,0
>   Final image : 400x400x400 Mean=0.551 Sigma=5.36
>      Sampling : 0.62 A/pixel   Origin = 9.53674e-007,9.53674e-007,9.53674e-007
> 
> E:\>proc3d tmv.map tmv3.mrc scale=3.4 clip=680,680,680
> EMAN 1.9 ($Date: 2009/02/18 05:12:22 $).
> Run 'proc3d help' for detailed help.
> Original image : 200x200x200 Mean=0.551 Sigma=5.74 Min=-26.3 Max=46.9
>      Sampling : 1.24 A/pixel   Origin = 0,0,0
> -------------------------------------------
> When scale=2 and clip=400,400,400, it worked.
> However scale=3.4 and clip=680,680,680, a error occured.
> the tmv.map file was downloaded from
> site:ftp://emfile.protein.osaka-u.ac.jp/emdb/structures/EMD-1730/map/emd_1730.map.gz.
> 
> Anyone have ever met similar cases and has solutions?
> I would be appreciated of you!
> -- 
> Regards!
> C.J.
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