[3dem] CEMOVIS

[Cedric Bouchet-Marquis]

Hi 

 

You can definitely use samples frozen in planchets for CEMOVIS. You'll have
to get some of the sample out of the planchet and mount it on a pin using
some cryo glue made of : ethanol: 2-propanol (2:3)

The only drawback here is that you'll have to cut at lower temps ~ -160 C
where samples break more easily and you'll probably get also more cutting
artifacts (crevasses in particular).

 

Ref: A cryoglue to mount vitreous biological specimens for
cryoultramicrotomy at 110K. Richter 1994

For more ref on that method, look at Al-Amoudi A. and Zuber B. work.

 

Cheers 

 

cedric

 

 

From: 3dem-bounces at ncmir.ucsd.edu [mailto:3dem-bounces at ncmir.ucsd.edu] On
Behalf Of Yi, Hong
Sent: Thursday, July 21, 2011 11:26 AM
To: 3dem at ncmir.ucsd.edu
Subject: [3dem] CEMOVIS

 

Dear All:

 

As far as I know, people usually freeze samples in a copper tube when doing
CEMOVIS.  We have a RMC HPM 010 so can not use a tube holder.  If we freeze
samples in a planchet, can it be used for CEMOVIS?  If yes, how should we
mount samples on our Leica UC6/FC6? 

 

Thank you all for your help.

 

Hong

 

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