[3dem] help on FSC plot

Sacha De Carlo sachadecarlo at yahoo.com
Thu Jan 20 10:25:09 PST 2011


See, I knew it... Pawel Penczek did :)
[Thanks Pawel].

Check out the Meth. in Enzymology volumes on image processing:

http://www.ncbi.nlm.nih.gov/pubmed/20888958

Best,
S.



================================================
Sacha De Carlo, Assistant Professor
Chemistry Department, Marshak Science Building &
New York Structural Biology Center
The City College of New York
Convent Ave & 138th Street
New York, NY 10031
(212) 650-6070
e-mail: sdecarlo at ccny.cuny.edu
URL: http://www.planetesacha.com
================================================

--- On Thu, 1/20/11, XIM9 at pitt.edu <XIM9 at pitt.edu> wrote:

From: XIM9 at pitt.edu <XIM9 at pitt.edu>
Subject: [3dem] help on FSC plot
To: 3dem at ncmir.ucsd.edu
Date: Thursday, January 20, 2011, 12:11 PM

Dear all,

I determined the resolution with the FSC plot. However, the FSC values
didn't drop down to the bottom, but stop at FSC=0.3, then increased. Does
anybody know why and how to fix it?

Two 3D volumes for FSC calculation were computed from two randomly
selected half of data set using BP 32F, SPIDER program.


Thanks,
Xin Meng


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