[3dem] Cytoskeleton

Guenter Resch guenter.resch at imba.oeaw.ac.at
Sat Feb 26 01:12:35 PST 2011

Hi Marcia,

> Does anyone have experience about how free cells that had their
> plasma membrane extracted with detergents  can be "glued" to a glass  
> coverslip (or other substratum) for SEM observation?

I guess you are referring to suspended cells that were extracted while
not attached?

I have no experience with this kind of model, but you might want to try
poly-L-lysine coated coverslips, those are quite sticky ...



Dr. Guenter Resch, IMP-IMBA-GMI Electron Microscopy Facility
Email: guenter.resch at imba.oeaw.ac.at; Web: http://cores.imp.ac.at/em
Phone: +43 (1) 79044-4250; Fax/Voice Mail: +43 (1) 79044-224250
Post: Dr. Bohr-Gasse 3, 1030 Vienna, Austria, European Union

More information about the 3dem mailing list