[3dem] Cytoskeleton

[Guenter Resch]

Hi Marcia,

> Does anyone have experience about how free cells that had their
> plasma membrane extracted with detergents  can be "glued" to a glass  
> coverslip (or other substratum) for SEM observation?

I guess you are referring to suspended cells that were extracted while
not attached?

I have no experience with this kind of model, but you might want to try
poly-L-lysine coated coverslips, those are quite sticky ...

Best,

	Guenter

-- 
Dr. Guenter Resch, IMP-IMBA-GMI Electron Microscopy Facility
Email: guenter.resch at imba.oeaw.ac.at; Web: http://cores.imp.ac.at/em
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