[3dem] ask for collaboration

Sacha De Carlo sachadecarlo at yahoo.com
Wed Oct 13 08:17:34 PDT 2010


you can attach DNA to the SWNT using streptavidine-biotine, then use any DNA binding protein (like RNA polymerase, as an example) to bind to the short DNA template, thus to the SWNT.

Best of luck,
- S

Sacha De Carlo, Assistant Professor
Chemistry Department, Marshak Science Building &
New York Structural Biology Center
The City College of New York
Convent Ave & 138th Street
New York, NY 10031
(212) 650-6070
e-mail: sdecarlo at ccny.cuny.edu
URL: http://www.planetesacha.com

--- On Wed, 10/13/10, Zunfeng Liu <liuz2 at chem.leidenuniv.nl> wrote:

From: Zunfeng Liu <liuz2 at chem.leidenuniv.nl>
Subject: [3dem] ask for collaboration
To: "3dem 3dem" <3dem at ucsd.edu>
Date: Wednesday, October 13, 2010, 10:09 AM

Dear All:

  I am looking for collaboration.

  My project is using SWNT-streptavidin and graphene-streptavidin complex to capture the big protein complex and characterize the structure using cryo-EM single particle analysis. The advantage of using SWNT or graphene is that the purification can be handled very quick and the unstable protein complex can be maintained.

   Now I am looking for a big protein used to check the validity of this method. And then I will purify the protein complex for 3D reconstruction.

   If the protein has biotin or strep-tag on it, it will be perfect!

   Thank you very much in advance!

Sincerely yours,



Zunfeng Liu

Faculteit/onderdeel: Wiskunde en Natuurwetenschappen, Leiden Institute of
Chemistry, LIC/Biophysical Structural Chemistry
Gorlaeus Laboratoria
Einsteinweg 55
2333 CC Leiden
Tel: +31 (0)71 5274801
email: liuz2 at chem.leidenuniv.nl; liuzunfeng at gmail.com

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