[3dem] Cryo-EM size limit

[R.I.Koning at lumc.nl]

Dear Reza,
 
Considering the on-going discussion I would additionally like to point out that there is a difference between 2D imaging of protein structures using cryo-EM and getting a succesfull 15 angstrom 3D reconstruction using single particle imaging and reconstruction techniques.
 
A successful reconstruction of small particles (lets say <300 kDa) depends  on the shape of the particle and also on when you consider it to be successful and what feature you want to resolve. Therefore it is difficult to get a practical limit. 
 
I wonder what the smallest particle is that has been resolved using singel particle cryo electron microscopy to 15 angstrom until now. Does anybody know? That might give you a practical answer to your question.
 
Regards,
 
Roman
 
Roman I. Koning, Ph.D.
Leiden University Medical Center
Department of Cell Biology
Section Electron Microscopy
Postal Zone S1-P
P.O.Box 9600
2300 RC, Leiden
The Netherlands
 
visiting adress: 
Einthovenweg 20,
Building 2, zone S1-P, room R-90-20
 
tel (31) 71 526 9296
fax (+31) 71 526 8270
r.i.koning at lumc.nl
 
 


-----Original Message-----
From: 3dem-bounces at ncmir.ucsd.edu [mailto:3dem-bounces at ncmir.ucsd.edu] On
Behalf Of Reza Khayat
Sent: Wednesday, October 27, 2010 12:34 PM
To: 3dem at ucsd.edu
Subject: [3dem] Cryo-EM size limit

Hi,
    I was wondering if anyone had an opinion on what the smallest  
macromolecular sample (in size or mass) could be for successful  
single particle cryo-EM and image reconstruction?  I'm hoping for a  
resolution better than 15Å.  This would be without the use of phase  
plate technology.

Thanks,
Reza

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